Eiko Nakahira and Shigeki Yuasa Neuronal generation, migration, and differentiation in the mouse hippocampal primoridium as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation The Journal of Comparative Neurology�

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(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer�

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Combined acupuncture and sonoporation for intradermal gene delivery

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Sonoporation or ultrasound-mediated cell membrane permeabilisation is proving to be an effective alternative to viral gene transfer [1]. Because of its non-invasive nature sonoporation also offers a number of advantages over alternative non-viral�

Combined acupuncture and sonoporation for intradermal gene delivery

CUY21 Publication List, Electrode Recommendations and Protocol Information

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LTM-1000 : Laser Thermal Microinjector

Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables�

LTM-1000 : Laser Thermal Microinjector

A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the hole�

Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)

4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms. Remove agarose gel immediately and wash it in Tyrode's solution. Then take out the tissues from the gel and wash them well in Tryode's solution. The tissues�

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Gene transfer into embryonic brains using in utero electroporation technique

The NEPA21 has been used in dozens of published applications. There are a few important details to manage for this system, so we encourage you to thoroughly review the topics on this page as they relate to your lab’s experiments. If you have any questions�

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old�

Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

Gene transfer into embryonic brains using in utero electroporation technique

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Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)

Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

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Gene transfer into embryonic brains using in utero electroporation technique

Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)

Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage

Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural�

Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage

Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Protocol for in vivo electroporation into mouse and rat retina

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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Electroporation-mediated gene transfer system applied to cultured CNS neurons

(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of�

Electroporation-mediated gene transfer system applied to cultured CNS neurons

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Electroporation for mammalian embryos in the whole embryo culture system

(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system. Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the�

Electroporation for mammalian embryos in the whole embryo culture system

(A) EGFP-expression vector was electroporated into E11.5 rat telencephalon. The electroporated embryo was cultured in the whole embryo culture system (WEC). (B) 24 hours after electroporation, EGFP-expression was specifically detected at the dorsal part�

Electroporation for mammalian embryos in the whole embryo culture system

Knock-down by transfection of shRNA expression vector by electroporation

(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression�

Knock-down by transfection of shRNA expression vector by electroporation

Direct Gene Transfer into Mature Seeds via Electroporation

Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis�

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In vivo gene transfer into the adult honeybee brain by using electroporation

C) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic�

In vivo gene transfer into the adult honeybee brain by using electroporation

Direct Gene Transfer into Mature Seeds via Electroporation

In vivo gene transfer into the adult honeybee brain by using electroporation

a) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic�

In vivo gene transfer into the adult honeybee brain by using electroporation

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ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao�

.Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

Gene transfer into embryonic brains using in utero electroporation technique

Electroporation for mammalian embryos in the whole embryo culture system

Electroporation-mediated gene transfer system applied to cultured CNS neurons

Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

Knock-down by transfection of shRNA expression vector by electroporation

Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)

Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage

In vivo gene transfer into the adult honeybee brain by using electroporation

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NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

LTM-1000 : Laser Thermal Microinjector

NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

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Microscopical demonstration of the interaction of a. SDM202 and b. SDM302 microbubbles with DNA. Pictures were taken on white (left) light and at 518 nm and 605 nm excitation and emission wavelengths, respectively (right) ( Nomikou et al., 2011�

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Cell Image Results Primary Mouse Microglia cells Viability 65% Transfection Efficiency 23%

Primary - Mouse Microglia cells

Cell Image Results Primary Mouse Microglia cells Viability 65% Transfection Efficiency 23%

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. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21_Super_Electro-Cell_Fusion_Generator_for_hybridoma_production_and_nuclear_transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

NEPA21_v_CUY21EDIT_CUY21SC_Amaxa_BTXECM830

- You will clearly see the following major differences depicted on the graphical illustration of the delivered electrical energy. These differences each individually have a marked impact on the quality and results of your experiments�

NEPA21_v_CUY21EDIT_CUY21SC

NEPA21_v_CUY21EDIT_CUY21SC_Amaxa_BTXECM830

Chick and Quail Embryo (in ovo) - Neural Tube, Mesencephalon, Diencephalon (HH* Stage 10

Chicken and Quail Embryo (In Ovo) - Neural Tube, Mesencephalon, Diencephalon (HH* Stage 10)

Direct Gene Transfer into Mature Seeds via Electroporation

The first method involves injecting a pair of needle electrodes CUY560-5/-10 into the muscle above skin. Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time. In our opinion�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

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Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral�

The chapter presents in step-by-step detail (for both Mouse and Rat) a new technique called ‘Technique for Animal Knockout system by Electroporation’ (TAKE Method), which produces genome-edited rodents by direct introduction of engineered endonucleases�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Organoid Electroporation Email

With this market-leading control and (user-independent) reproducibility of the technique, it is now possible to apply electroporation techniques to applications previously considered too sensitive for electroporation methodologies. One such application�

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- When placing the embryos in the electrode, try to place them in the middle of the electrode. As EP produces bubbles, if the embryos are placed too close the electrode, the embryos disappear (and are vapourised) with the bubbles that come off the�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

High-performance and reliable site-directed in vivo genetic manipulation of mouse and rat brain by in utero electroporation with a triple-electrode probe, Joanna Szczurkowska, Andrzej W. Cwetsch, Marco dal Maschio, Diego Ghezzi, Gian Michele�

However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700P_L type-electrodes in combination with the following electrodes�

However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes�

Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production

Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target. This control is generated via unique electroporation pulse-output configurations�

Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production

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Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production

Free-NEPA21-Demo-and-Trial-:-Zygote-Electroporation-for-Transgenic-Animal-Production

Test Zygote email

Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita�

bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan�

Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui�

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Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production

Test Page Jump

In Vitro Cell Transfection Database: NEPA21

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Hints and Tips iGONAD Procedure

Recent progress in of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal Nucleic Acids Delivery (GONAD); a novel in vivo genome�

Hints and Tips iGONAD Procedure

Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production

Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production

test 1

Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel 3, Chaowan Guo 1, Yasukazu Daigaku 4, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato�

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Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production

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Organoid Electroporation Email

Organoid Electroporation Email

With particle bombardment, the transformants are often contaminated by bacteria and/or fungi, making it necessary to wash and isolate single cells using glass capillaries under a microscope (Pringsheim, 1946). This operation sometimes results in additional�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

The efficiency of transformation is not very high; the average transformation efficiency following selection in 0.3 μgml-1 phleomycin was 5.5 9 10-6 cells or 0.03 transformants μg–1 DNA (Abe et al., 2011). Therefore, many cells must be prepared�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Kotaro Kiga, Xin-Ee Tan, Rodrigo Ibarra-Chávez, Shinya Watanabe, Yoshifumi Aiba, Yusuke Sato'o, Feng-Yu Li, Teppei Sasahara, Bintao Cui, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Aa Haeruman Azam, Masato Suzuki, José R�

ELEPO21 Results

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Test spacing 2

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ELEPO21 Results

Test spacing 2

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