Plant Protoplast Fusion by Electroporation

The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure�

Plant Protoplast Fusion may be performed by Electroporation. Per this new technique, genes are delivered into a seed by electroporation. (This new electroporation method has been developed and patented by Dr. Hagio of the Japanese Institute of�

Plant Protoplast Fusion By Electro Cell Fusion

3. Unlike the CUY21 range of electroporators, the LF101 and LF201 do not have an automatic safety cut-off switch. As compared with that of CUY21 range, the DC pulse length of both the LF101 and LF201 is very short (usec). In addition, unlike the�

Adult Mouse and Rat (In vivo) - Vessel

For Adult Mouse and Rat (in vivo) Vessel applications we recommend the following electrode: CUY250

Photo CUY250-0.8

Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate�

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Vacuum & Electrical Pulse Method for Plant Seed Electroporation

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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CUY21 Publication List, Electrode Recommendations and Protocol Information

The NEPA21 Electroporator is based on the same platform and core technology that made the CUY21EDIT and CUY21SC devices leaders in their class. The NEPA21 can do in one device what each of the EDIT and SC did individually, i.e., it combines BOTH the�

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer�

Combined acupuncture and sonoporation for intradermal gene delivery

Sonoporation or ultrasound-mediated cell membrane permeabilisation is proving to be an effective alternative to viral gene transfer [1]. Because of its non-invasive nature sonoporation also offers a number of advantages over alternative non-viral�

Combined acupuncture and sonoporation for intradermal gene delivery

Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages

This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information. �

Combined acupuncture and sonoporation for intradermal gene delivery

IntroductionSonoporation or ultrasound-mediated cell membrane permeabilisation is proving to be an effective alternative to viral gene transfer [1]. Because of its non-invasive nature sonoporation also offers a number of advantages over alternative�

Combined acupuncture and sonoporation for intradermal gene delivery

Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture

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Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture

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Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture

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Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture

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Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture

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Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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Combined acupuncture and sonoporation for intradermal gene delivery

LF201 Publication Listing for Cell Fusion and Nuclear Transfer

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LF201 Publication Listing

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LF201 Publication Listing for Cell Fusion and Nuclear Transfer

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LF201 Publication Listing for Cell Fusion and Nuclear Transfer

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LF201 Publication Listing

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LF201 Publication Listing for Cell Fusion and Nuclear Transfer

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Improve Electroporation Transfection Efficiency with the CU902 Polarity Exchanger

During electroporation, DNA naturally moves to and accumulates at the positive (+) electrode pole (similar to the process in electrophoresis). However, such accumulation (if not addressed) adversely impacts on electroporation transfection efficiency�

Improve Electroporation Transfection Efficiency with the CU902 Polarity Exchanger

Plant Protoplast Fusion By Electro Cell Fusion

A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the hole�

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Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage

The resistance of an EP target varies according to physical experimental factors such as the volume of the sample, the buffer used and the distance between the electrodes. Fluctuations in resistance impact on delivered current values and this negatively�

Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural�

Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage

Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Electroporation-mediated gene transfer system applied to cultured CNS neurons

(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of�

Electroporation-mediated gene transfer system applied to cultured CNS neurons

Misexpression of the gene of interest by in ovo electroporation

Mechanisms of brain regionalization and neural circuit formation have been studied by misexpression of transcription factors (En1/2, Pax2/5/6, Otx2, Gbx2), secreted factors (Fgf, Shh, semaphoring), signal transduction molecule (Ras, Sprouty2) and receptors�

Misexpression of the gene of interest by in ovo electroporation

Gene transfer into muscle by In Vivo electroporation

Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (�

Gene transfer into muscle by In Vivo electroporation

Chick embryo Electroporation using News culture gastrula

GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as�

Chick embryo Electroporation using News culture gastrula

Chick embryo Electroporation using News culture (gastrula)

Chick embryo Electroporation using News culture gastrula

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Vacuum & Electrical Pulse Method for Plant Seed Electroporation

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Plant Protoplast Fusion by Electroporation

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Improve Electroporation Transfection Efficiency with the CU902 Polarity Exchanger

Improve Electroporation Transfection Efficiency with the CU902 Polarity Exchanger

Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages

CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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Connector Cables for CUY21 Electroporator and Electrodes

If one uses the tweezers-type electrode with the ECM830 the C117 is not required. The C117 is used to hook a needle-type electrode such as the CUY611 series and the CUY613 series. Tweezers-type electrodes such as the CUY650 series have the required�

CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages

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Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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CUY21 Publication List, Electrode Recommendations and Protocol Information

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Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages

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Low-cost solution to increase Electroporation Transfection Efficiency

Simple, low-cost solution to increase Electroporation Transfection Efficiency

Low-cost solution to increase Electroporation Transfection Efficiency

Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages

CUY21 Publication List, Electrode Recommendations and Protocol Information

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Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages

LF201 Publication Listing for Cell Fusion and Nuclear Transfer

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LF201 Publication Listing for Cell Fusion and Nuclear Transfer

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ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao�

Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies�

Electroporation-mediated gene transfer in the adult rat brain

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Electroporation-mediated gene transfer system applied to cultured CNS neurons

Electroporation-mediated gene transfer in the adult rat brain

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Gene transfer into muscle by In Vivo electroporation

Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

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Misexpression of the gene of interest by in ovo electroporation

Misexpression of the gene of interest by in ovo electroporation

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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NEP21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

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NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

NEP21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

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Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables�

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NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

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NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations

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Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems

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New Range of Microbubbles

Microscopical demonstration of the interaction of a. SDM202 and b. SDM302 microbubbles with DNA. Pictures were taken on white (left) light and at 518 nm and 605 nm excitation and emission wavelengths, respectively (right) ( Nomikou et al., 2011�

New Range of Microbubbles

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ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

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.Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21_Super_Electro-Cell_Fusion_Generator_for_hybridoma_production_and_nuclear_transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

In a test tube spontaneous fusing of two different somatic cells occurs less than 1 in 1,000,000 times. Though this is good if you are a living organism, it is sometimes desirable to fuse cells for specialized applications. Historically, a chemical�

ECFG21 Super Electro-Cell Fusion Generator

Somatic cell fusion is an involved technique traditionally used for making hybridomas for monoclonal antibodies. This technique fuses an immortal myleloma cell line with a spleen-derived antibody-producing B-cell. The hybridoma cell line produced will�

ECFG21 Super Electro-Cell Fusion Generator

Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Additionally, inactivated viruses have been used for the same effect . More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

ECFG21_Super_Electro-Cell_Fusion_Generator_for_hybridoma_production_and_nuclear_transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Electrofusion was performed using the ECFG21 and LF201 Electro Cell Fusion Generator (SONIDEL/Nepa Gene) and the CUY497P2 MS Stand Model Chamber Type Platinum Electrode, L80mm x W2mm x H5mm, 0.8ml (SONIDEL/Nepa Gene), (Fig. 1). The electrofusion buffer�

Electrofusion was performed using the LF201 Electro Cell Fusion Generator (Nepa Gene, Japan) and the CUY497P2 MS Stand Model Chamber Type Platinum Electrode, L80mm x W2mm x H5mm, 0.8ml (Nepa Gene, Japan), (Fig. 1). The electrofusion buffer�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Electrofusion was performed using the LF201 Electro Cell Fusion Generator (SONIDEL/Nepa Gene) and the CUY497P2 MS Stand Model Chamber Type Platinum Electrode, L80mm x W2mm x H5mm, 0.8ml (SONIDEL/Nepa Gene), (Fig. 1). The electrofusion buffer�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

NEPA21_Algae-Transformation_without_cell-wall_removal

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NEPA21_Algae-Transformation_without_cell-wall_removal

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ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

. Traditionally, a chemical method such as PEG has been used to fuse cells together (to an equivalent 2 or 3 orders of magnitude). Inactivated viruses have also been used for the same effect. More recently, methodologies combining successive AC and�

NEPA21_v_CUY21EDIT_CUY21SC_Amaxa_BTXECM830

- You will clearly see the following major differences depicted on the graphical illustration of the delivered electrical energy. These differences each individually have a marked impact on the quality and results of your experiments�

NEPA21_v_CUY21EDIT_CUY21SC

NEPA21_v_CUY21EDIT_CUY21SC_Amaxa_BTXECM830

Vacuum & Electrical Pulse Method for Plant Seed Electroporation

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Vacuum & Electrical Pulse Method for Plant Seed Electroporation

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

Vacuum & Electrical Pulse Method for Plant Seed Electroporation

Vacuum & Electrical Pulse Method for Plant Seed Electroporation

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Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral�

Single-Cell/Micro-Particle_Transfer-Micro_Pick_and_Place_(MPP)_System_Configurations

The PicoPipet is an electronic ultra-small-pipetting device that comprises an electro-osmotic pump in the pipette part. The special pump can easily control microfluidic flows without pulsation. The weight of the pipette part is just 10 g and has been�

Single-Cell/Micro-Particle_Transfer-Micro_Pick_and_Place_(MPP)_System_Configurations

The PicoPipet is an electronic ultra-small-pipetting device that has an electroosmotic pump in its pipette part. The special pump can easily control microfluidic flows without pulsation. The weight of the pipette part is just 10 g, and that enables precise�

Single-Cell/Micro-Particle_Transfer-Micro_Pick_and_Place_(MPP)_System_Configurations

- MPP System Brochurehttp://www.sonidel.com/Single-Cell_Micro-Particle_Transfer/Micro_Pick_and_Place_System_Brochure.pdf...http://www.sonidel.com/Single-Cell_Micro-Particle_Transfer/Micro_Pick_and_Place_System_Brochure.pdfMPP-300 Micro Pick and Place�

Single-Cell/Micro-Particle_Transfer-Micro_Pick_and_Place_(MPP)_System_Configurations

Vacuum & Electrical Pulse Method for Plant Seed Electroporation

Adult Mouse and Rat (In vivo) - Liver

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Adult Mouse and Rat (In vivo) - Liver

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Oviduct EP with the NEPA21 - GONAD Technique

For your further information, Dr. Masato Otsuka also uses our CUY52P2.5X4 electrode for his GONAD method. The electrode surface is concaved (bowl-like shape) and thus makes it easier to grasp/hold the oviduct. The electrode is to Dr. Ohtsuka’s�

Oviduct EP with the NEPA21 - GONAD Technique

ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

In Utero Electrode Recommendation

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In Utero Electrode Recommendation

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ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer

With this market-leading control and (user-independent) reproducibility of the technique, it is now possible to apply electroporation techniques to applications previously considered too sensitive for electroporation methodologies. One such application�

Oviduct EP with the NEPA21 - GONAD Technique

The NEPA21 both uniquely monitors in real-time the delivered energy (Joules) and enables the researcher to matches the energy required for the different ‘poration’ and ‘payload driving’ events. (Please email sales@sonidel.com to request a�

The NEPA21 has been used in dozens of published applications. There are a few important details to manage for this system, so we encourage you to thoroughly review the topics on this page as they relate to your lab’s experiments. If you have any questions�

Support Page

Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita�

bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan�

Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui�

Hints and Tips iGONAD Procedure

Recent progress in of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal Nucleic Acids Delivery (GONAD); a novel in vivo genome�

Hints and Tips iGONAD Procedure

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- The finer control over the delivered energy available with the NEPA21 offers specific and important advantages for organoid electroporation. As the thrust of NEPA21 protocols is to minimise delivered energy, this means that the targets are electroporated�

The finer control over the delivered energy available with the NEPA21 offers specific and important advantages for organoid electroporation. As the thrust of NEPA21 protocols is to minimise delivered energy, this means that the targets are electroporated�

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With particle bombardment, the transformants are often contaminated by bacteria and/or fungi, making it necessary to wash and isolate single cells using glass capillaries under a microscope (Pringsheim, 1946). This operation sometimes results in additional�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug plasmid DNA linearized in 1.5 uL TE buffer, and 30.5 uL C medium�

The efficiency of transformation is not very high; the average transformation efficiency following selection in 0.3 μgml-1 phleomycin was 5.5 9 10-6 cells or 0.03 transformants μg–1 DNA (Abe et al., 2011). Therefore, many cells must be prepared�

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials) 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

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Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

Materials : 1.5 ug plasmid DNA linearized (The total volume is 40uL/2mm gap cuvettes: 8 uL Opti-MEM, 1.5 ug �

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