New Systems for Gene Transfer

Long-Term in vivo Sonoporation Transfection in mouse hind leg at day 150

Long-Term Ultrasound expression

In vivo gene transfer using the SONIDEL Ultrasound Transfection platform

In vitro Sonoporation Expression of EGFP following transfection of Hela Cells

In Vitro Sonoporation Expression of Hela Cells

In vitro gene transfer using the SONIDEL Ultrasound Transfection platform

The SONIDEL Limited Ultrasound Transfection Platform comprises:

SONIDEL SP100 Sonoporator
SONIDEL MB101 Microbubble for gene transfer - reversible cell membrane permeabilisation
SONIDEL STK10 Positive Control Kit for ultrasound mediated gene transf

Publications and Protocols

Sonoporation Protocols & Device Recommendation

The listed Sonoporation publications are right up to date to August 2009.

The listed Electroporation, Electro Cell Fusion and Plant Protoplast Fusion publications and protocols specifically cite the CUY21 EDIT and CUY21 SC range of electroporators and the LF101 and LF201 range of cell fusion devices.

Electroporation

Electrode & Protocol Recommendations for CUY21 EDIT and CUY21 SC Electroporators:

Mouse & Rat:

Further Electroporation Electrode Recommendations:

Mouse Embryo (In Utero)

Mouse and Rat (Whole Embryo Culture)

Adult Mouse and Rat (In Vivo)

Newborn Mouse and Rat (In Vivo)

Adult Mouse, Rat, Rabbit, Dog and Monkey (DNA Vaccine)

Adult Mouse, Rat, Rabbit and Dog (In Vivo)

Skin (Epidermis, Dermis, Hypodermis)

Chick Embryo (Organ Culture)

Digestive Organ Epithelial Cell (HH stage 27-28)

Chick Embryo (New Culture)

Gastrulation, Neural Plate (HH Stage 3-7)

Chick and Quail Embryo (In Ovo)

Neural Tube, Mesencephalon Diencephalon (HH* stage 10)

Bee (In Vivo)

Brain

Silkworm

Xenopus Embryo (In Vivo)

Stage 12.5

Fish

Tissue Fragment (Ex Vivo)

Plant (In Vivo)

Seed Embryo

New Vacuum and Electroporation Method for Plant Seed (Rice, Wheat, Barley), Egg and Microbe

Vacuum & Electroporation Protocol & Electrode Recommendation:

More

N: Negative control, --: Buffer only, V: Vacuum, E: Electroporation, VE: Vacuum and Electroporation (the new method)

Advantages of Plant Seed Vacuum and Electroporation Method

No Need for Tissue Culture
No Particular Know-How or Skill required
Large reduction in handling and labour time

Simple
Efficient
Low-Cost

GUS gene expression reference (Wheat)

Electro Cell Fusion (ECF)

Electrode and Protocol Recommendations for LF101 and LF201:

Bovine Cloning
Cat Cloning
Dog Cloning
Mouse Embryo Cloning
Porcine Cloning
Rabbit Cloning
Controlling Electrofusion
Plant Protoplast Fusion by Electro Cell Fusion
Producing Antibodies - comparison of Electro Cell Fusion (LF201) and PEG Reagent
ECF leads to double or triple efficiency
The results of ECF are more stable

Cables

for CUY21 EDIT, CUY21 SC, LF101 and LF201:

Sonidel Limited

achieving ultrasound-mediated transient cell membrane permeabilisation
specialising in the development of novel Ultrasound technologies for Gene Therapy applications
delivering standardisation and measureable control to the sonoporation market
developed the World�s First positive control (STK10) for U/S mediated Gene Transfer
developed the first low cost commercially available sonoporator (SP100) range designed to deliver consistent & reproducible output

View Our Top Sellers

SP100 MB101 Microbubble STK10 Transfection Kit CUY21 EDIT CUY21 SC Tissue/Embryo Electrode Mouse Embryo (Eye) Electrode Mouse Electrodes Chick Electrodes		LF201 LF101 OCH01 Cell Holder

Featured Devices

Product Code: OCH01
Desc:	Cell Holder
Applic:	Electroporation and Sonoporation

Product Code: SONIDEL STK10
Desc:	Ultrasound Transfection Positive Control
Applic:	Sonoporation Transfection Kit

Product Code: SONIDEL SP100
Desc:	Sonoporator
Applic:	(hover for application)Application: Delivery of plasmids to cells and tissues for gene therapy-based applications and studies. Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy. Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues. Delivery of agents to cells to study metabolic effects.

Featured Electrodes

Product Code: CUY900-25-5-4: Adherent Cell Electrode in well plate

Application:
Electrodes for Electroporation

Description:
Adherent cell electrode, 25mmφ, 5 plates, 4mm gap

Product Code: CUY497P1: Small Volume

Application:
Electrodes for Electro Cell Fusion

Description:
CUY497P1: Small Volume Micro Slide Stand Model Chamber Platinum Electrode - L80 x W2 x H5 mm, 1mm gap, Volume 0.8ml - Connected with CUY499L

Product Code: CUY202

Application:
Electrodes for Electroporation

Description:
Electrode guide

Product Code: CUY557-1

Application:
Electrodes for Electroporation

Description:
Stainless Steel, Parallel fixed needle electrodes, 1mm tip length, 1mm gap, 0.2mmf

Product Code: CUY500G0.5

Application:
Electrodes for Electro Cell Fusion

Description:
Gold wire electrodes on microscope slide, 0.5mm gap, 3/pkg

Product Code: CUY671P1

Application:
Electrodes for Electroporation

Description:
Platinum parallel fixed needle electrodes, 0.5mmf, 1mm gap